• Product name
  • Description
    Rabbit polyclonal to UBPY/USP8
  • Host species
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1068 and 1118 of Human UBPY/USP8 (NP_005145.3).

  • Positive control
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab103524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 1 MIT domain.
      Contains 1 rhodanese domain.
      Contains 1 USP domain.
    • Domain
      The MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
      The rhodanese domain is sufficient for RNF41-binding.
    • Post-translational
      Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
      Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
    • Cellular localization
      Cytoplasm. Nucleus. Endosome membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 8 antibody
      • FLJ34456 antibody
      • hUBPy antibody
      • HumORF8 antibody
      • KIAA0055 antibody
      • MGC129718 antibody
      • SPG59 antibody
      • Ubiquitin carboxyl-terminal hydrolase 8 antibody
      • Ubiquitin isopeptidase Y antibody
      • ubiquitin specific peptidase 8 antibody
      • Ubiquitin thioesterase 8 antibody
      • Ubiquitin-specific-processing protease 8 antibody
      • UBP8_HUMAN antibody
      • UBPY antibody
      • Usp8 antibody
      see all


    • Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. (1) ab103524 at at 3 µg/mg lysate. (2) IgG control.


    ab103524 has not yet been referenced specifically in any publications.

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