Product nameAnti-UCHL1+3 antibody
DescriptionRabbit polyclonal to UCHL1+3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant human protein purified from E.coli (Tagged-UCHL1)
- SK-N-MC cell, IMR-32 cell, Mouse brain and Rat brain lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, HEPES, 0.01% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab75275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 25 kDa.|
RelevanceThe two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220 amino acids that share more than 40% amino acid sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD).
- Ubiquitin C terminal esterase L1 antibody
- Ubiquitin carboxyl terminal esterase L1 antibody
- Ubiquitin carboxyl terminal esterase L3 antibody
All lanes : Anti-UCHL1+3 antibody (ab75275) at 1/2000 dilution
Lane 1 : SK-N-MC cell lysate
Lane 2 : IMR-32 cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
ab75275 has not yet been referenced specifically in any publications.