Recombinant Anti-UQCRH antibody [EPR9039(B)] (ab134949)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9039(B)] to UQCRH
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UQCRH antibody [EPR9039(B)]
See all UQCRH primary antibodies -
Description
Rabbit monoclonal [EPR9039(B)] to UQCRH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human UQCRH aa 50-150. The exact sequence is proprietary.
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Positive control
- Human colon and thyroid carcinoma tissues; HL60 cells; HL60, fetal muscle, fetal liver, fetal brain, fetal heart, fetal kidney, and Human spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 3.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9039(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c. -
Cellular localization
Mitochondrial. Mitochondrion inner membrane. -
Database links
- Entrez Gene: 7388 Human
- Entrez Gene: 66576 Mouse
- Entrez Gene: 366448 Rat
- Omim: 613844 Human
- SwissProt: P07919 Human
- SwissProt: P99028 Mouse
- SwissProt: Q5M9I5 Rat
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Alternative names
- Complex III subunit 6 antibody
- Complex III subunit VIII antibody
- Cytochrome b c1 complex subunit 6 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling UQCRH with ab134949 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embeddedHuman thyroid carcinoma tissue labelling UQCRH with ab134949 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-UQCRH antibody [EPR9039(B)] (ab134949) at 1/1000 dilution
Lane 1 : Fetal muscle lysate
Lane 2 : Fetal liver lysate
Lane 3 : HL60 cell lysate
Lane 4 : Fetal brain lysate
Lane 5 : Fetal heart lysate
Lane 6 : Fetal kidney lysate
Lane 7 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa -
Immunohistochemical analysis using ab134949 showing positive staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis using ab134949 showing positive staining in Normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab134949 has been referenced in 7 publications.
- Robinson DRL et al. Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain. Front Cell Dev Biol 10:786268 (2022). PubMed: 35300415
- Vidali S et al. Characterising a homozygous two-exon deletion in UQCRH: comparing human and mouse phenotypes. EMBO Mol Med 13:e14397 (2021). PubMed: 34750991
- Luo Y et al. UQCRH downregulation promotes Warburg effect in renal cell carcinoma cells. Sci Rep 10:15021 (2020). PubMed: 32929120
- Zhang S et al. Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly. Nat Commun 11:1312 (2020). PubMed: 32161263
- Jing YY et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978
- Song W et al. Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue. PLoS Biol 17:e2006571 (2019). PubMed: 30653498
- Park ER et al. The mitochondrial hinge protein, UQCRH, is a novel prognostic factor for hepatocellular carcinoma. Cancer Med 6:749-760 (2017). WB . PubMed: 28332314