Anti-USP10 antibody (ab72486)
Key features and details
- Rabbit polyclonal to USP10
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-USP10 antibody
See all USP10 primary antibodies -
Description
Rabbit polyclonal to USP10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 87 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 87 kDa. |
Target
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Function
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peptidase C19 family. USP10 subfamily. -
Post-translational
modificationsPhosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus. -
Cellular localization
Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM. - Information by UniProt
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Database links
- Entrez Gene: 9100 Human
- Omim: 609818 Human
- SwissProt: Q14694 Human
- Unigene: 136778 Human
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Alternative names
- Deubiquitinating enzyme 10 antibody
- KIAA0190 antibody
- MGC124997 antibody
see all
Images
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All lanes : Anti-USP10 antibody (ab72486) at 1/1000 dilution
Lane 1 : HEK293
Lane 2 : HEK293/USP10
Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (15)
ab72486 has been referenced in 15 publications.
- Bhattacharya U et al. The deubiquitinase USP10 protects pancreatic cancer cells from endoplasmic reticulum stress. NPJ Precis Oncol 6:93 (2022). PubMed: 36543867
- Wang X et al. The deubiquitinase USP10 regulates KLF4 stability and suppresses lung tumorigenesis. Cell Death Differ 27:1747-1764 (2020). PubMed: 31748695
- Zhu H et al. USP10 Promotes Proliferation of Hepatocellular Carcinoma by Deubiquitinating and Stabilizing YAP/TAZ. Cancer Res 80:2204-2216 (2020). PubMed: 32217697
- Kim K et al. Prognostic significance of USP10 and p14ARF expression in patients with colorectal cancer. Pathol Res Pract 216:152988 (2020). PubMed: 32362421
- Xie W et al. Auto-ubiquitination of NEDD4-1 Recruits USP13 to Facilitate Autophagy through Deubiquitinating VPS34. Cell Rep 30:2807-2819.e4 (2020). PubMed: 32101753