Recombinant Anti-Ube1L antibody [EPR4270] (ab108929)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4270] to Ube1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Ube1L antibody [EPR4270]
See all Ube1L primary antibodies -
Description
Rabbit monoclonal [EPR4270] to Ube1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HepG2, HL60, HeLa, Jurkat, and human fetal liver cell lysate. GST tagged Recombinant human UBA7(Ube1L) protein. ICC/IF: HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4270 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
Tissue specificity
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. - Information by UniProt
-
Database links
- Entrez Gene: 7318 Human
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
-
Alternative names
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
see all
Images
-
All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa -
All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
-
Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab108929 has not yet been referenced specifically in any publications.