Recombinant HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
See all Ubiquitin primary antibodies -
Description
HRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Di-Ubiquitin/Ub2 (K27 Linked).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17034 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)
- Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237254)
- Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237255)
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
Notes |
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WB
1/1000. |
Target
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Relevance
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains. -
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- Entrez Gene: 192255 Rat
- SwissProt: P0CG47 Human
- SwissProt: P0CG48 Human
- SwissProt: P62979 Human
- SwissProt: P62987 Human
- SwissProt: P62988 Human
see all -
Alternative names
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
see all
Images
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This blot was produced using a 4-12% Bis-Tris gel under the MES buffer system. The gel was run at 200V for 35 mins. before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237256 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Samples:
Lane 1. Di-Ubiquitin/Ub2 (Full Length) (20ng)
Lane 2. Di-Ubiquitin/Ub2 (K11 Linked) (20ng)
Lane 3. Di-Ubiquitin/Ub2 (K27 Linked) (20ng)
Lane 4. Di-Ubiquitin/Ub2 (K29 Linked) (20ng)
Lane 5. Di-Ubiquitin/Ub2 (K33 Linked ) (20ng)
Lane 6. Di-Ubiquitin/Ub2 (K48 Linked) (20ng)
Lane 7. Di-Ubiquitin/Ub2 (K6 Linked) (20ng)
Lane 8. Di-Ubiquitin/Ub2 (K63 Linked) (20ng)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237256 has not yet been referenced specifically in any publications.