Product nameUniversal HIER antigen retrieval reagent (10X)
Abcam’s Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. Antibody titers are doubled or tripled when compared to citrate buffer, pH 6.0. Universal HIER antigen retrieval reagent (10X) incorporates a color-coded high temperature pH indicator solution, allowing the end-user to check that the solution is at the correct dilution and pH by visual inspection. This product is specially formulated for superior pH stability at high temperatures in order to help prevent the possibility of losing pH-sensitive antigens. Universal HIER antigen retrieval reagent (10X) is non-toxic, non-flammable, odorless and sodium azide- and thimerosal-free.
Ab208572 is the recommended HIER antigen retrieval reagent for
PD-L1 RabMAb (clone 28-8, Ab205921).
The Universal HIER antigen retrieval reagent is a buffered solution that is intended for laboratory use in heat induced antigen retrieval of formalin-fixed paraffin-embedded (FFPE) tissues for immunohistochemistry (IHC) procedures. The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Tested applicationsSuitable for: IHCmore details
Storage instructionsStore at room temperature. Please refer to protocols.
Components 100 ml Universal HIER antigen retrieval reagent 1 x 100ml
Our Abpromise guarantee covers the use of ab208572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration.|
Human tonsil stained with PD-L1 RabMAb antibody (clone 28-8, Ab205921) using Universal HIER antigen retrieval reagent (ab208572).
Human tonsil stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).
Human melanoma stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).
This product has been referenced in:
- Wang W et al. Immunoclassification characterized by CD8 and PD-L1 expression is associated with the clinical outcome of gastric cancer patients. Oncotarget 9:12164-12173 (2018). Read more (PubMed: 29552300) »
- Drakes ML et al. Stratification of ovarian tumor pathology by expression of programmed cell death-1 (PD-1) and PD-ligand- 1 (PD-L1) in ovarian cancer. J Ovarian Res 11:43 (2018). Read more (PubMed: 29843813) »