Recombinant
RabMAb

Anti-Uroplakin III antibody [EPR3612(2)] (ab150409)

Overview

  • Product name
    Anti-Uroplakin III antibody [EPR3612(2)]
    See all Uroplakin III primary antibodies
  • Description
    Rabbit monoclonal [EPR3612(2)] to Uroplakin III
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Uroplakin III aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human bladder lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3612(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 31 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence.
    • Tissue specificity
      Expressed in ureter.
    • Involvement in disease
      Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
    • Sequence similarities
      Belongs to the uroplakin-3 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER).
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC119178 antibody
      • OTTHUMP00000028951 antibody
      • UP III antibody
      • UP3a antibody
      • UPIII antibody
      • UPIIIA antibody
      • UPK 3 antibody
      • UPK 3A antibody
      • UPK3 antibody
      • UPK3A antibody
      • UPK3A_HUMAN antibody
      • Uroplakin 3A antibody
      • Uroplakin III antibody
      • Uroplakin-3a antibody
      • Uroplakin3A antibody
      • UroplakinIII antibody
      see all

    Images

    • Anti-Uroplakin III antibody [EPR3612(2)] (ab150409) at 1/1000 dilution + Human bladder
      lysate
      at 10 µg

      Predicted band size: 31 kDa
      Observed band size: 47 kDa (why is the actual band size different from the predicted?)

    References

    ab150409 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Marmoset (common) Tissue sections (Bladder)
    Specification
    Bladder
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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