Recombinant Anti-Uroplakin III antibody [EPR3612(2)] (ab150409)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3612(2)] to Uroplakin III
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Uroplakin III antibody [EPR3612(2)]
See all Uroplakin III primary antibodies -
Description
Rabbit monoclonal [EPR3612(2)] to Uroplakin III -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Uroplakin III aa 50-150. The exact sequence is proprietary.
-
Positive control
- Human bladder lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3612(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 31 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 31 kDa). |
Target
-
Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
Tissue specificity
Expressed in ureter. -
Involvement in disease
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
Sequence similarities
Belongs to the uroplakin-3 family. -
Cellular localization
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
-
Database links
- Entrez Gene: 7380 Human
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
-
Alternative names
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
Images
-
Anti-Uroplakin III antibody [EPR3612(2)] (ab150409) at 1/1000 dilution + Human bladder
lysate
at 10 µg
Predicted band size: 31 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab150409 has not yet been referenced specifically in any publications.