Overview

  • Product name
    Anti-VAC14 antibody - N-terminal
    See all VAC14 primary antibodies
  • Description
    Rabbit polyclonal to VAC14 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VAC14 aa 125-159 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q08AM6

  • Positive control
    • Human kidney tissue; Human spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab210940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 88 kDa.
IHC-P 1/25. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the VAC14 family.
    Contains 6 HEAT repeats.
  • Domain
    The C-terminal domain (residues 523-782) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.
  • Cellular localization
    Endosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • ArPIKfyve antibody
    • FLJ10305 antibody
    • FLJ36622 antibody
    • FLJ46582 antibody
    • MGC149815 antibody
    • MGC149816 antibody
    • OTTHUMP00000174908 antibody
    • Protein VAC14 homolog antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 1 antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 2 antibody
    • Tax1 binding protein 2 antibody
    • Tax1-binding protein 2 antibody
    • TAX1BP2 antibody
    • TRX antibody
    • VAC 14 antibody
    • vac14 antibody
    • Vac14 homolog (S. cerevisiae) antibody
    • Vac14 homolog antibody
    • VAC14_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded Human kidney tissue labeling VAC14 with ab210940 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

  • Anti-VAC14 antibody - N-terminal (ab210940) at 1/2000 dilution + Human spleen lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 88 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab210940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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