Anti-VGF antibody (ab115609)
Key features and details
- Rabbit polyclonal to VGF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-VGF antibody
See all VGF primary antibodies -
Description
Rabbit polyclonal to VGF -
Host species
Rabbit -
Specificity
The immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
17 amino acid peptide near the carboxy terminus of the Human VGF.
-
Positive control
- Human Brain Cortex tissue; Human Brain tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 0.99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. -
Tissue specificity
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. -
Post-translational
modificationsMultiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure. -
Cellular localization
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. - Information by UniProt
-
Database links
- Entrez Gene: 7425 Human
- Entrez Gene: 381677 Mouse
- Entrez Gene: 29461 Rat
- Omim: 602186 Human
- SwissProt: O15240 Human
- SwissProt: P20156 Rat
- Unigene: 587325 Human
-
Alternative names
- NERP-1 antibody
- NERP-2 antibody
- Nerve growth factor inducible antibody
see all
Images
-
ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
All lanes : Human Brain tissue lysate
Predicted band size: 67 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab115609 has been referenced in 1 publication.
- Marwitz S et al. Epigenetic modifications of the VGF gene in human non-small cell lung cancer tissues pave the way towards enhanced expression. Clin Epigenetics 9:123 (2017). PubMed: 29209432