Anti-Vitellogenin antibody [ND-3G6] (ab36794)

Overview

  • Product name
    Anti-Vitellogenin antibody [ND-3G6]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [ND-3G6] to Vitellogenin
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    The monoclonal antibody ND-3G6 binds with high affinity to vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica). The antibody also cross-reacts with vitellogenin from other bird species, including American kestrel (Falco sparverius), zebra finch (Taeniopygia guttata) and domestic chicken (Gallus gallus). The degree of cross-reactivity differs between species and with the methods employed.
  • Immunogen

    Mixture of Vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    ND-3G6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/100 - 1/1000.
WB 1/50 - 1/500.

Target

  • Relevance
    Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
  • Cellular localization
    Secreted
  • Alternative names
    • Vg antibody
    • VTG antibody
    • VTG1 antibody

Images

  • Coating: Purified Vtg (2 µg/ml) or egg yolk (y; diluted 1:500). Primary antibody: ND-3G6
  • ab36794 staining Vitellogenin in chicken ovary sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde, blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab36794 at a 1/20 dilution for 20 hours at 4°C. The secondary used was a 5nm Gold conjugated goat anti-rabbit polyclonal used at a 1/40 dilution. Counterstained with HandE.

    See Abreview

References

ab36794 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (ovaries)
Specification
ovaries
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Peter Wooding

Verified customer

Submitted Sep 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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