Anti-Von Willebrand Factor antibody (ab9378)
Key features and details
- Rabbit polyclonal to Von Willebrand Factor
- Suitable for: IHC-P, Flow Cyt, WB, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Von Willebrand Factor antibody
See all Von Willebrand Factor primary antibodies -
Description
Rabbit polyclonal to Von Willebrand Factor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Factor VIII related antigen isolated from human plasma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3 -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (8) |
1/50 - 1/100.
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa).
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/50 - 1/100. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa). |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Database links
- Entrez Gene: 7450 Human
- Entrez Gene: 22371 Mouse
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q8CIZ8 Mouse
- Unigene: 440848 Human
- Unigene: 22339 Mouse
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Alternative names
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Von Willebrand Factor with ab9378.
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Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 309 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Many bands of varying sizes can be seen on Western blots, perhaps indicating differential processing by ADAMTS13 and other enzymes -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab9378 has been referenced in 28 publications.
- Lan G et al. Astrocytic VEGFA: An essential mediator in blood-brain-barrier disruption in Parkinson's disease. Glia 70:337-353 (2022). PubMed: 34713920
- Woods VMA et al. Targeting transgenic proteins to alpha granules for platelet-directed gene therapy. Mol Ther Nucleic Acids 27:774-786 (2022). PubMed: 35116189
- Guo S et al. Effects of diagnostic ultrasound with cRGD-microbubbles on simultaneous detection and treatment of atherosclerotic plaque in ApoE-/- mice. Front Cardiovasc Med 9:946557 (2022). PubMed: 35935617
- Zhang Q et al. Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages. Nat Commun 12:6867 (2021). PubMed: 34824203
- Sudhadevi T et al. Hyperoxia-induced S1P1 signaling reduced angiogenesis by suppression of TIE-2 leading to experimental bronchopulmonary dysplasia. Cell Biochem Biophys 79:561-573 (2021). PubMed: 34176100