Anti-XBP1 antibody (ab37152)
Key features and details
- Rabbit polyclonal to XBP1
- Suitable for: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-XBP1 antibody
See all XBP1 primary antibodies -
Description
Rabbit polyclonal to XBP1 -
Host species
Rabbit -
Specificity
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1. -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
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Positive control
- WB: HepG2 cell lysate, A549 cell lysate. IHC-P: Human liver and non-lesional skin tissue; Mouse testis and spleen tissue, rat liver tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (12) |
Use a concentration of 5 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 20028872
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ICC |
Use a concentration of 10 µg/ml.
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IHC (PFA fixed) |
Use a concentration of 20 µg/ml.
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WB | (11) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).
Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information. Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
ICC/IF | (3) |
Use a concentration of 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 20028872 |
ICC
Use a concentration of 10 µg/ml. |
IHC (PFA fixed)
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information. Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. -
Involvement in disease
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
Alternative names
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using ab37152 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Lane 1 : Anti-XBP1 antibody (ab37152) at 0.25 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 0.5 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
All lanes : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry of mouse testis cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry of mouse spleen cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry of mouse pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry of human pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunocytochemistry of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml.
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ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
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ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
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Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (146)
ab37152 has been referenced in 146 publications.
- Metzing UB et al. Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis. Sci Rep 12:504 (2022). PubMed: 35017615
- Shen J et al. Homocysteine facilitates endoplasmic reticulum stress and apoptosis of hepatocytes by suppressing ERO1α expression via cooperation between DNMT1 and G9a. Cell Biol Int 46:1236-1248 (2022). PubMed: 35347798
- Liu Z et al. XBP1 deficiency promotes hepatocyte pyroptosis by impairing mitophagy to activate mtDNA-cGAS-STING signaling in macrophages during acute liver injury. Redox Biol 52:102305 (2022). PubMed: 35367811
- Malik A et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
- Zhang Y et al. Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis. Mediators Inflamm 2022:8007078 (2022). PubMed: 35497095