Anti-ZCWPW2 antibody (ab126482)
Key features and details
- Rabbit polyclonal to ZCWPW2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCWPW2 antibody -
Description
Rabbit polyclonal to ZCWPW2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: TATPDESEEG HGEEINMGEK LSKCSPEAPA GSLFENHYEE DYLVIDGIKL KAGECIEDIT NKFKEIDALM SEF, corresponding to amino acids 284-356 of Human ZCWPW2.
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Positive control
- Human adrenal gland tissue; RT4, U251 MG and Human liver lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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WB |
1/250 - 1/500. Predicted molecular weight: 41 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
WB
1/250 - 1/500. Predicted molecular weight: 41 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 CW-type zinc finger.
Contains 1 PWWP domain. - Information by UniProt
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Database links
- Entrez Gene: 152098 Human
- SwissProt: Q504Y3 Human
- Unigene: 510635 Human
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Alternative names
- ZCPW2_HUMAN antibody
- ZCW2 antibody
- ZCWPW2 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, vesicles and cytoskeleton (microtubules). Recommended concentration of ab126482 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab126482 at a 1/500 dilution staining ZCWPW2 in paraffin embedded Human adrenal gland tissue by immunohistochemistry.
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All lanes : Anti-ZCWPW2 antibody (ab126482) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 41 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126482 has not yet been referenced specifically in any publications.