Recombinant Anti-ZNRF2 antibody [EPR11399(B)] (ab156011)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11399(B)] to ZNRF2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ZNRF2 antibody [EPR11399(B)]
See all ZNRF2 primary antibodies -
Description
Rabbit monoclonal [EPR11399(B)] to ZNRF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human ZNRF2. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, Human fetal brain and Neuro-2a lysates, Human ovarian carcinoma tissue, Human testis tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11399(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. -
Tissue specificity
Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is required for E3 ligase activity. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Endosome membrane. Lysosome membrane. Cell junction > synapse > presynaptic cell membrane. Present in presynaptic plasma membranes in neurons. - Information by UniProt
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Database links
- Entrez Gene: 223082 Human
- Entrez Gene: 387524 Mouse
- Entrez Gene: 362367 Rat
- Omim: 612061 Human
- SwissProt: Q8NHG8 Human
- SwissProt: Q71FD5 Mouse
- Unigene: 487869 Human
- Unigene: 286149 Mouse
see all -
Alternative names
- E3 ubiquitin-protein ligase ZNRF2 antibody
- Protein Ells2 antibody
- RING finger protein 202 antibody
see all
Images
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All lanes : Anti-ZNRF2 antibody [EPR11399(B)] (ab156011) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Neuro-2a lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab156011 has been referenced in 1 publication.
- Lee CY et al. The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation. Nat Commun 8:15502 (2017). PubMed: 28593998