Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)
Key features and details
- Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes
- Suitable for: IHC-P
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] -
Description
Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Tissue, cells or virus corresponding to Zebrafish Zebrafish Gut Secretory Cell Epitopes. Tissue/cell preparation: Lysate of zebrafish intestine.
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Positive control
- IHC: Zebrafish Larvae (5 days)
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
FIS 2F11/2 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 µg/ml.
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Notes |
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IHC-P
Use a concentration of 0.5 µg/ml. |
Images
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IHC image of Zebrafish Gut Secretory Cell Epitopes staining in a section of formalin-fixed paraffin-embedded Zebra fish Larvae (5 days) performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab71286, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab71286 has been referenced in 30 publications.
- Zhao C et al. Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome. Hepatology 75:567-583 (2022). PubMed: 34569629
- He J et al. DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration. NPJ Regen Med 7:21 (2022). PubMed: 35351894
- Morales RA et al. Interleukin-10 regulates goblet cell numbers through Notch signaling in the developing zebrafish intestine. Mucosal Immunol 15:940-951 (2022). PubMed: 35840681
- Cao Z et al. Shikonin Inhibits Fin Regeneration in Zebrafish Larvae. Cells 11:N/A (2022). PubMed: 36291055
- Cheung Y et al. Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage. J Pediatr Surg 56:352-359 (2021). PubMed: 32709532