Anti-Zinc finger antiviral protein antibody (ab154680)
Key features and details
- Rabbit polyclonal to Zinc finger antiviral protein
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Zinc finger antiviral protein antibody
See all Zinc finger antiviral protein primary antibodies -
Description
Rabbit polyclonal to Zinc finger antiviral protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Zinc finger antiviral protein aa 401-721 (internal sequence).
Database link: Q7Z2W4 -
Positive control
- A431 whole cell lysate; A431 cells; Human hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/5000 - 1/20000. Predicted molecular weight: 101 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 101 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Induces an innate immunity to viral infections by preventing the accumulation of viral RNAs in the cytoplasm. Seems to recruit the RNA processing exosome to degrade the target RNAs. Inhibits alphavirus and filovirus replication. -
Sequence similarities
Contains 4 C3H1-type zinc fingers.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain. -
Domain
The second and fourth zinc fingers are involved in binding to specific viral RNAs. -
Cellular localization
Cytoplasm. Nucleus. Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 56829 Human
- Omim: 607312 Human
- SwissProt: Q7Z2W4 Human
- Unigene: 133512 Human
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Alternative names
- ADP ribosyltransferase diphtheria toxin like 13 antibody
- ARTD13 antibody
- FLB6421 antibody
see all
Images
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Anti-Zinc finger antiviral protein antibody (ab154680) at 1/10000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 101 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Zinc finger antiviral protein with ab154680 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling Zinc finger antiviral protein with ab154680 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab154680 has been referenced in 10 publications.
- Sungsuwan S et al. Zinc-finger antiviral protein-mediated inhibition of porcine epidemic diarrhea virus growth is antagonized by the coronaviral nucleocapsid protein. Front Microbiol 13:975632 (2022). PubMed: 36160209
- Goonawardane N et al. Association of Zinc Finger Antiviral Protein Binding to Viral Genomic RNA with Attenuation of Replication of Echovirus 7. mSphere 6:N/A (2021). PubMed: 33408233
- Chiang C et al. The Small t Antigen of JC Virus Antagonizes RIG-I-Mediated Innate Immunity by Inhibiting TRIM25's RNA Binding Ability. mBio 12:N/A (2021). PubMed: 33849980
- Paparisto E et al. Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein. Cells 10:N/A (2021). PubMed: 34572049
- Sertkaya H et al. Minimal impact of ZAP on lentiviral vector production and transduction efficiency. Mol Ther Methods Clin Dev 23:147-157 (2021). PubMed: 34703838
- Ficarelli M et al. CpG Dinucleotides Inhibit HIV-1 Replication through Zinc Finger Antiviral Protein (ZAP)-Dependent and -Independent Mechanisms. J Virol 94:N/A (2020). PubMed: 31748389
- Li MMH et al. Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP). J Virol 93:N/A (2019). PubMed: 31118263
- Ficarelli M et al. KHNYN is essential for the zinc finger antiviral protein (ZAP) to restrict HIV-1 containing clustered CpG dinucleotides. Elife 8:N/A (2019). PubMed: 31284899
- Odon V et al. The role of ZAP and OAS3/RNAseL pathways in the attenuation of an RNA virus with elevated frequencies of CpG and UpA dinucleotides. Nucleic Acids Res 47:8061-8083 (2019). PubMed: 31276592
- Li MM et al. TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). PLoS Pathog 13:e1006145 (2017). WB . PubMed: 28060952