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What would be a suitable positive control for this product 14-3-3 (phospho S) antibody (0014-33)?
Asked on Jun 01 2007
Thank you for your enquiry. For immunohistochemistry paraffin-embedded sections, human breast carcinoma was used in as control. For Western Blot QC, lysates were prepared from A431 cells, untreated or calyculin A-treated. A431 cells are a human epithelial carcinoma cell line. Calyculin treatment greatly increased the expression of the 14-3-3 Binding Motif, phosphorylated (Ser) in a number of different proteins. Abcam lists this product ab7909, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, which could serve as a decent positive control for Western Blotting.
Answered on Jun 01 2007