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I have read the two papers you mentioned about, but I still do not think they are very convincing. First, the GnRH receptor is different from HCG receptor either in function or in structure. Even the existence of GnRH receptor in HepG2 cells does not mean there are HCG receptors in them, too. And, although there are some changes when HepG2 cells being cultured with hCG, there is no direct proof that the HCG receptor is definitely there. While in the paper I mentioned( Shan LX, Hardy MP Endocrinology. 1992 Sep;131(3):1107-14. ( PMID: 1505454, ), the authors did the Northern blot analysis showing there was no HCGR mRNA in HepG2 cells. I think this is a more solid proof. I want to know whether your company has ever tested the Anti-HCGR antibody on HepG2 cells . Because in my experiment, the Western blot showed some bands in HepG2 lane, but not at 79KDa band weight which is showed in your protocol. I do not know whether it is caused by the lack of specifity of this antibody. If you have tested the antibody in HepG2 cells, I want to know whether the results are the same. If you have not tested it, I do not think it is a good idea to list the HepG2 cells in the positive control before more convincing results been published. Thank you very much! Best wishes,
Asked on Feb 17 2012
Thank you for taking the time to look into this and for your response.
We have not tested this in HepG2 cells and the positive control recommendations were based on the literature. I thank you for your recommendation with this and have removed HepG2 from the controls.
Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Feb 17 2012