Key features and details
- Rabbit polyclonal to A RAF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-A RAF antibody
See all A RAF primary antibodies
DescriptionRabbit polyclonal to A RAF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human A RAF aa 150-200. The exact sequence is proprietary. (NP_001645.1).
Database link: P10398
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225628 was affinity purified using an epitope specific to A RAF immobilized on solid support.
Our Abpromise guarantee covers the use of ab225628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue specificityPredominantly in urogenital tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
- Information by UniProt
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
All lanes : Anti-A RAF antibody (ab225628) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Exposure time: 3 minutes
A RAF was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225628 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225628 at 1 µg/ml.
Lane 1: ab225628 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225628 has not yet been referenced specifically in any publications.