Product nameAnti-A RAF antibody
See all A RAF primary antibodies
DescriptionRabbit polyclonal to A RAF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human A RAF aa 150-200. The exact sequence is proprietary. (NP_001645.1).
Database link: P10398
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225628 was affinity purified using an epitope specific to A RAF immobilized on solid support.
Our Abpromise guarantee covers the use of ab225628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue specificityPredominantly in urogenital tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
- Information by UniProt
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
All lanes : Anti-A RAF antibody (ab225628) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Exposure time: 3 minutes
A RAF was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225628 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225628 at 1 µg/ml.
Lane 1: ab225628 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab225628 has not yet been referenced specifically in any publications.