Overview

  • Product name

  • Description

    Rabbit polyclonal to A RAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human A RAF aa 150-200. The exact sequence is proprietary. (NP_001645.1).
    Database link: P10398

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
  • Tissue specificity

    Predominantly in urogenital tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • A raf 1 antibody
    • A Raf proto oncogene serine/threonine protein kinase antibody
    • ARAF 1 antibody
    • Araf antibody
    • ARaf proto oncogene serine/threonine protein kinase antibody
    • ARAF_HUMAN antibody
    • ARAF1 antibody
    • Oncogene Araf1 antibody
    • Oncogene PKS2 antibody
    • PKS 2 antibody
    • PKS antibody
    • PKS2 antibody
    • Proto oncogene Pks antibody
    • Proto-oncogene A-Raf antibody
    • Proto-oncogene A-Raf-1 antibody
    • Proto-oncogene Pks antibody
    • RAFA 1 antibody
    • RAFA1 antibody
    • Ras binding protein DA Raf antibody
    • Serine/threonine-protein kinase A-Raf antibody
    • v raf murine sarcoma 3611 viral oncogene homolog antibody
    • v raf murine sarcoma 3611 viral oncogene homolog 1 antibody
    • v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody
    see all

Images

  • All lanes : Anti-A RAF antibody (ab225628) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 68 kDa


    Exposure time: 3 minutes
  • A RAF was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225628 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225628 at 1 µg/ml.

    Lane 1: ab225628 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab225628 has not yet been referenced specifically in any publications.

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