Overview

  • Product name

  • Description

    Mouse polyclonal to A1CF/ACF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human A1CF/ACF aa 1-594.
    Database link: NP_620311.1

  • Positive control

    • Placenta or HeLa whole cell lysate (ab150035).
  • General notes

     This product was previously labelled as A1CF

     

Properties

Applications

Our Abpromise guarantee covers the use of ab89050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.

Target

  • Function

    Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
  • Tissue specificity

    Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Domain

    The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
  • Cellular localization

    Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1CF antibody
    • A1CF_HUMAN antibody
    • ACF antibody
    • ACF64 antibody
    • ACF65 antibody
    • Apo B RNA editing protein antibody
    • Apobec 1 complementation factor antibody
    • Apobec 1 complementation factor (ACF) (ASP) antibody
    • APOBEC 1 stimulating protein antibody
    • APOBEC1 complementation factor antibody
    • APOBEC1 stimulating protein antibody
    • APOBEC1-stimulating protein antibody
    • APOBEC1CF antibody
    • ASP antibody
    • MGC163391 antibody
    • RP11-564C4.2 antibody
    see all

Images

  • Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg

    Predicted band size: 65 kDa

  • Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size: 65 kDa

  • All lanes : Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml

    Lane 1 : A1CF/ACF-transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab89050. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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