Overview

  • Product name

    Anti-A2B5 antibody [105] - BSA and Azide free
    See all A2B5 primary antibodies
  • Description

    Mouse monoclonal [105] to A2B5 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Tissue, cells or virus corresponding to Chicken A2B5.

  • Positive control

    • Type II astrocytes, human neural progenitors.
  • General notes

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab53521.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab175579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells.
  • Cellular localization

    Cell surface

Images

  • This flow cytometry data was generated using the same antibody clone in a different buffer formulation (ab53521).

    Overlay histogram showing SH-SY5Y cells stained with ab53521 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

ab175579 has not yet been referenced specifically in any publications.

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