Product nameAnti-A2BP1/Fox1/RBFOX1 antibody
See all A2BP1/Fox1/RBFOX1 primary antibodies
DescriptionRabbit polyclonal to A2BP1/Fox1/RBFOX1
Tested applicationsSuitable for: WB, ELISA, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide corresponding to Human A2BP1/Fox1/RBFOX1 aa 1-50 (N terminal).
Database link: NP_001135805
- MCF7 cell lysate
This product was previously labelled as A2BP1 / Fox1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:62500.
|IHC-FoFr||Use at an assay dependent concentration.|
FunctionRNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Tissue specificityPredominantly expressed in muscle and brain.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 2BP1 antibody
- A2 BP1 antibody
- A2BP 1 antibody
Anti-A2BP1/Fox1/RBFOX1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Sellier C et al. rbFOX1/MBNL1 competition for CCUG RNA repeats binding contributes to myotonic dystrophy type 1/type 2 differences. Nat Commun 9:2009 (2018). Read more (PubMed: 29789616) »
- Chen PF et al. RBFOX1 and RBFOX2 are dispensable in iPSCs and iPSC-derived neurons and do not contribute to neural-specific paternal UBE3A silencing. Sci Rep 6:25368 (2016). WB . Read more (PubMed: 27146458) »