Recombinant Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Product name

Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50]
See all A2BP1/Fox1/RBFOX1 primary antibodies
Description

Rabbit monoclonal [EPR23627-50] to A2BP1/Fox1/RBFOX1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, ICC, Flow Cyt (Intra), IPmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human A2BP1/Fox1/RBFOX1 aa 1-200. The exact sequence is proprietary.
Database link: Q9NWB1
Positive control
- WB: Human brain; Mouse brain, brain cortex and skeletal muscle; Rat brain and brain cortex, U-2 OS; Neuro-2a; L6 lysates. IHC-Fr: Mouse cerebrum; Rat cerebrum. ICC: Rat primary neural/glia; Neuro-2a; L6; U-2 OS cells; mouse primary neural/glia cells. Flow Cyt: U-2 OS; Neuro-2a cells. IP: Human brain; Mouse tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23627-50
Isotype

IgG
Research areas

Alternative Versions
- Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
Compatible Secondaries
Dyes/Markers
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-MAP2 antibody [HM-2] (ab11267)

Our Abpromise guarantee covers the use of ab254412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 42 kDa.
IHC-Fr		1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
ICC		1/100.
Flow Cyt (Intra)		1/500.
IP		1/30.

Application notes

Is unsuitable for IHC-P.

Function

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Tissue specificity

Predominantly expressed in muscle and brain.
Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.
Cellular localization

Nucleus. Cytoplasm.
Target information above from: UniProt accession Q9NWB1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 54715 Human
- Entrez Gene: 268859 Mouse
- Entrez Gene: 302920 Rat
- Omim: 605104 Human
- SwissProt: Q9NWB1 Human
- SwissProt: Q9JJ43 Mouse
- Unigene: 459842 Human
- Unigene: 370334 Mouse
- Unigene: 92540 Rat
see all
Alternative names
- 2BP1 antibody
- A2 BP1 antibody
- A2BP 1 antibody
- A2BP antibody
- A2BP1 antibody
- Ataxin 2 binding protein 1 antibody
- Ataxin-2-binding protein 1 antibody
- Ataxin2 binding protein 1 antibody
- FOX 1 antibody
- Fox-1 homolog A antibody
- fox-1-like RNA-binding protein 1 antibody
- Hexaribonucleotide binding protein 1 antibody
- Hexaribonucleotide-binding protein 1 antibody
- HRNBP 1 antibody
- HRNBP1 antibody
- RBFOX1 antibody
- RFOX1_HUMAN antibody
- RNA binding protein fox-1 homolog 1 antibody
- RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
- RNA binding protein, fox-1 homolog (C. elegans) 1 antibody
see all

Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

All lanes : Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse brain cortex tissue lysate
Lane 5 : Mouse skeletal muscle tissue lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Rat brain tissue lysate
Lane 8 : Rat brain cortex tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 42 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: liver(PMID:12574126, PMID:16260614, PMID:20724578, PMID:20724578, PMID:10814712).

Exposure time: Lanes 1-2: 3 minutes; Lanes 3-6: 3 seconds; Lanes 7-8: 6 seconds.
Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor®594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution.
Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

A2BP1/Fox1/RBFOX1 was immunoprecipitated from 0.35 mg Human brain tissue lysate 10 ug with ab254412 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human brain tissue lysate 10 ug

Lane 2: ab254412 IP in Human brain tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab254412 in human brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds.
Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

All lanes : Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412) at 1/2000 dilution

Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 3 : L6 (rat skeletal muscle myoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 103 seconds
Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) antibody at 1/1000 (Green). Confocal image showing nuclear and cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 (1.144 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

A2BP1/Fox1/RBFOX1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab254412 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10 ug

Lane 2: ab254412 IP in Mouse brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254412 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.
Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (11.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 (1.144 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L6 cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on rat liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on mouse liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab254412? Please let us know so that we can cite the reference in this datasheet.

ab254412 has not yet been referenced specifically in any publications.

Recombinant Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Dyes/Markers

Isotype control

Related Products

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (0)

Customer reviews and Q&As