Key features and details
- Mouse polyclonal to A3H
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-A3H antibody
DescriptionMouse polyclonal to A3H
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human A3H aa 1-182.
- Human colon tissue lysate and transfected 293 cell line lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab73055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceARP10 belongs to the cytidine and deoxycytidylate deaminase family. It is a poorly expressed deaminase that is ineffective at inhibiting retroviral replication. ARP10 has DNA deaminase (cytidine deaminase) activity but is not able to mediate G-to-A hypermutation in newly synthesized retroviral DNA. It is present at low level in 293T cells. It is considered an unstable protein, suggesting that it may be degraded by the proteasome (at protein level). Weakly expressed in testis, ovary, fetal liver and skin. There are two named isoforms. ARP10 from old world monkeys has retained its antiviral activity, while it is lost in other primates. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.
Cellular localizationNucleus. Cytoplasm. Note: Mostly nuclear.
- A3H antibody
- APOBEC related protein 10 antibody
- APOBEC3H antibody
Anti-A3H antibody (ab73055) at 1/500 dilution + human colon tissue lysate at 25 µg
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted?
All lanes : Anti-A3H antibody (ab73055) at 1/500 dilution
Lane 1 : A3H transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab73055 has been referenced in 1 publication.
- Desimmie BA et al. APOBEC3 proteins can copackage and comutate HIV-1 genomes. Nucleic Acids Res 44:7848-65 (2016). PubMed: 27439715