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Phoned because of concern regarding the positive controls listed on the datasheet. According to published reference, HepG2 is a negative control.
Asked on Feb 16 2012
Thank you for contacting us. I was able to find the reference that you spoke of on the phone (ShanLX, HardyMP Endocrinology.1992 Sep;131(3):1107-14. (PMID:1505454,http://www.ncbi.nlm.nih.gov/pubmed/1505454). During my research for this question I was able to find a later paper in whichHepG2 lines are shown to haveGnRHreceptors,Pati D,Habibi HR.Endocrinology.1995 Jan;136(1):75-84.(PMID:7828560; ). A more recent paper, Jacobson HI, et.al.Adv Exp Med Biol.2008;617:477-84. (PMID: 18497072,http://www.ncbi.nlm.nih.gov/pubmed/18497072) use HepG2 culture with hCG as well. Is seems that the early information has been supplanted by this new evidence. Therefore I believe that the HepG2 cells should be a positive control for this product. If you have further concern I would recommend using A431 cell lysate (https://www.abcam.com/A431-Human-epithelial-carcinoma-cell-line-Whole-Cell-Lysate-ab7909.html) or H1299 cells. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 16 2012