- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-A4GNT antibody
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DescriptionGoat polyclonal to A4GNT
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Host speciesGoat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide:
C-PRTYRDLIKGPEGS
, corresponding to C terminal amino acids 317-330 of Human A4GNT (NP_057245.1). -
Positive control
- K562 cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab99187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
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FunctionNecessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
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Tissue specificityDetected in stomach and pancreas.
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PathwayProtein modification; protein glycosylation.
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Sequence similaritiesBelongs to the glycosyltransferase 32 family.
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DomainThe conserved DXD motif is involved in enzyme activity.
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Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 51146 Human
- SwissProt: Q9UNA3 Human
- Unigene: 278960 Human
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Alternative names
- 4-N-acetylglucosaminyltransferase antibody
- A4GCT_HUMAN antibody
- A4GNT antibody
see all
Images
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Western blot - Anti-A4GNT antibody (ab99187)Anti-A4GNT antibody (ab99187) at 1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
Protocols
References
ab99187 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"