Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab99187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
FunctionNecessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
Tissue specificityDetected in stomach and pancreas.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 32 family.
DomainThe conserved DXD motif is involved in enzyme activity.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 4-N-acetylglucosaminyltransferase antibody
- A4GCT_HUMAN antibody
- A4GNT antibody
Anti-A4GNT antibody (ab99187) at 1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab99187 has not yet been referenced specifically in any publications.