Anti-A4GNT antibody (ab99187)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-A4GNT antibody
See all A4GNT primary antibodies -
Description
Goat polyclonal to A4GNT -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide:
C-PRTYRDLIKGPEGS
, corresponding to C terminal amino acids 317-330 of Human A4GNT (NP_057245.1). -
Positive control
- K562 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab99187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. -
Tissue specificity
Detected in stomach and pancreas. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 32 family. -
Domain
The conserved DXD motif is involved in enzyme activity. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 51146 Human
- SwissProt: Q9UNA3 Human
- Unigene: 278960 Human
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Alternative names
- 4-N-acetylglucosaminyltransferase antibody
- A4GCT_HUMAN antibody
- A4GNT antibody
see all
Images
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Western blot - Anti-A4GNT antibody (ab99187)Anti-A4GNT antibody (ab99187) at 1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Protocols
Datasheets and documents
References
ab99187 has not yet been referenced specifically in any publications.
