Overview

  • Product name
    Anti-A549 antibody
  • Description
    Goat polyclonal to A549
  • Host species
    Goat
  • Specificity
    ab25816 recognises all A-549 cell lines tested. It may recognise proteins from other human cell lines.
  • Tested applications
    Suitable for: WB, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): Solubilized total A-549 cell proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: PBS, Sodium citrate
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa. Note: Reacts with more than 40 proteins by Western Blot of Heat/SDS/DTT solubilized A-549 cells.
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    A-549 cells are used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Cells are cytokeratin positive.
  • Alternative names
    • A 549 antibody
    • A-549 antibody
    • a549 antibody
    • type II pulmonary epithelial cel antibody

References

ab25816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam regarding ab25816.


I am sorry that you have been experiencing difficulties with this antibody in WB. In order to be able to help you further with this issue, I was hoping you could provide some additional information about your protocol and samples. Would you please provide a detailed summary of your sample preparation and WB protocol? An image would also be helpful. Once I have this information, I would be happy to offer some protocol suggestions or arrange for a solution per our Abpromise guarantee. Please also provide a lot number and order number if available.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions or concerns.

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Answer

Thank you for your reply.


I am sorry that you continue to experience difficulties with this antibody. The image is quite helpful, so thank you for sending that.


I am not sure what you were expecting to see from this antibody, however the datasheet outlines that the antibody will recognize more than 40 proteins by WB, including proteins from other human cell lines. The fact that it is not recognizing any protein from the E. coli lysate suggests that this antibody is functioning as expected.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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