Overview

  • Product name

  • Description

    Rabbit polyclonal to AACS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AACS aa 435-598.
    Database link: Q86V21

  • Positive control

    • WB: HEK-293 whole cell lysate. IHC-P: Human kidney tissue. IP: HEK-293 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab237802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 75 kDa.
IP 1/200 - 1/2000.
IHC-P 1/20 - 1/200.

Target

  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Acetoacetyl-CoA synthetase antibody
    • ACSF1 antibody
    • Acyl-CoA synthetase family member 1 antibody
    • Protein sur-5 homolog antibody

Images

  • Anti-AACS antibody (ab237802) at 1/500 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 75 kDa

  • Paraffin-embedded human kidney tissue stained for AACS using ab237802 at 1/100 dilution in immunohistochemical analysis.

  • AACS was immunoprecipitated from 500 µg HEK-293 (human epithelial cell line from embryonic kidney whole cell lysate with ab237802 at 1/200 dilution. 

    Lane 1: Rabbit control IgG IP in HEK-293 whole cell lysate.

    Lane 2: ab237802 IP in HEK-293 whole cell lysate.

    Lane 3: HEK-293 whole cell lysate 10 µg (Input).

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

References

ab237802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237802.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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