Anti-AACT antibody (ab127202)
- Datasheet
- References
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Overview
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Product name
Anti-AACT antibody
See all AACT primary antibodies -
Description
Rabbit polyclonal to AACT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human AACT aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127875) -
Positive control
- This antibody gave a positive signal in the following Tissue lysates: Human Spinal cord; Human Colon; Hu Plasma total protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab127202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
Tissue specificity
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
Involvement in disease
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 12 Human
- Entrez Gene: 100173860 Orangutan
- Omim: 107280 Human
- SwissProt: P01011 Human
- SwissProt: Q5R536 Orangutan
- Unigene: 534293 Human
- Unigene: 710488 Human
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Alternative names
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
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Images
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Western blot - Anti-AACT antibody (ab127202)All lanes : Anti-AACT antibody (ab127202) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lane 3 : Human Plasma Total Protein Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesAACT contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References
ab127202 has not yet been referenced specifically in any publications.
