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Anti-AACT antibody (ab127202)

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Western blot - Anti-AACT antibody (ab127202)

    Key features and details

    • Rabbit polyclonal to AACT
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human AACT protein (ab182695)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-AACT antibody
      See all AACT primary antibodies
    • Description

      Rabbit polyclonal to AACT
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Macaque monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Human AACT aa 150-250 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab127875)

    • Positive control

      • This antibody gave a positive signal in the following Tissue lysates: Human Spinal cord; Human Colon; Hu Plasma total protein.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Proteases
      • Cardiovascular
      • Blood
      • Serum Proteins
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Other proteases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human AACT protein (ab182695)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab127202 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

    Target

    • Function

      Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
    • Tissue specificity

      Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
    • Involvement in disease

      Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
    • Sequence similarities

      Belongs to the serpin family.
    • Domain

      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01011 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12 Human
      • Entrez Gene: 100173860 Orangutan
      • Omim: 107280 Human
      • SwissProt: P01011 Human
      • SwissProt: Q5R536 Orangutan
      • Unigene: 534293 Human
      • Unigene: 710488 Human
      • Alternative names

        • SERPINA3 antibody
        • AACT antibody
        • AACT_HUMAN antibody
        • ACT antibody
        • alpha 1 Antichymotrypsin antibody
        • Alpha-1-antichymotrypsin His-Pro-less antibody
        • Antichymotrypsin antibody
        • Cell growth inhibiting gene 24/25 protein antibody
        • Cell growth-inhibiting gene 24/25 protein antibody
        • GIG24 antibody
        • GIG25 antibody
        • Growth inhibiting protein 24 antibody
        • Growth inhibiting protein 25 antibody
        • MGC88254 antibody
        • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
        • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
        • Serine proteinase inhibitor clade A member 3 antibody
        • Serpin A3 antibody
        • Serpin family A member 3 antibody
        • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
        • SERPINA3 antibody
        see all

      Images

      • Western blot - Anti-AACT antibody (ab127202)
        Western blot - Anti-AACT antibody (ab127202)
        All lanes : Anti-AACT antibody (ab127202) at 1 µg/ml

        Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
        Lane 2 : Human colon tissue lysate - total protein (ab30051)
        Lane 3 : Human Plasma Total Protein Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 48 kDa
        Observed band size: 60 kDa why is the actual band size different from the predicted?
        Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 8 minutes


        AACT contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab127202? Please let us know so that we can cite the reference in this datasheet.

      ab127202 has not yet been referenced specifically in any publications.

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