Anti-AACT antibody (ab127202)

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Western blot - Anti-AACT antibody (ab127202)

    Key features and details

    • Rabbit polyclonal to AACT
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-AACT antibody
      See all AACT primary antibodies

    • Description

      Rabbit polyclonal to AACT

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Macaque monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide corresponding to Human AACT aa 150-250 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab127875)

    • Positive control

      • This antibody gave a positive signal in the following Tissue lysates: Human Spinal cord; Human Colon; Hu Plasma total protein.

    • General notes

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab127202 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

    Target

    • Function

      Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

    • Tissue specificity

      Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

    • Involvement in disease

      Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].

    • Sequence similarities

      Belongs to the serpin family.

    • Domain

      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • SERPINA3 antibody
        • AACT antibody
        • AACT_HUMAN antibody
        • ACT antibody
        • alpha 1 Antichymotrypsin antibody
        • Alpha-1-antichymotrypsin His-Pro-less antibody
        • Antichymotrypsin antibody
        • Cell growth inhibiting gene 24/25 protein antibody
        • Cell growth-inhibiting gene 24/25 protein antibody
        • GIG24 antibody
        • GIG25 antibody
        • Growth inhibiting protein 24 antibody
        • Growth inhibiting protein 25 antibody
        • MGC88254 antibody
        • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
        • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
        • Serine proteinase inhibitor clade A member 3 antibody
        • Serpin A3 antibody
        • Serpin family A member 3 antibody
        • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
        • SERPINA3 antibody
        see all

      Images

      • Western blot - Anti-AACT antibody (ab127202)
        Western blot - Anti-AACT antibody (ab127202)
        All lanes : Anti-AACT antibody (ab127202) at 1 µg/ml

        Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
        Lane 2 : Human colon tissue lysate - total protein (ab30051)
        Lane 3 : Human Plasma Total Protein Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 48 kDa
        Observed band size: 60 kDa why is the actual band size different from the predicted?
        Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 8 minutes


        AACT contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      References (0)

      Publishing research using ab127202? Please let us know so that we can cite the reference in this datasheet.

      ab127202 has not yet been referenced specifically in any publications.

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