Overview

  • Product name

  • Description

    Goat polyclonal to AACT
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human AACT aa 222-236 (internal sequence) (Cysteine residue). NP_001076.2.
    Sequence:

    C-DPQDTHQSRFYLSKK


    Database link: P01011

  • Positive control

    • WB: Human albumin-depleted serum and plasma lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificity

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in disease

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • alpha 1 Antichymotrypsin antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin family A member 3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Images

  • All lanes : Anti-AACT antibody (ab223226) at 0.3 µg/ml

    Lane 1 : Human albumin-depleted serum lysate (in RIPA buffer)
    Lane 2 : Human plasma lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223226 has not yet been referenced specifically in any publications.

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