RecombinantRabMAb

Recombinant Anti-AACT antibody [EPR14117(B)] (ab180492)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14117(B)] to AACT
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-AACT antibody [EPR14117(B)]
    See all AACT primary antibodies

  • Description

    Rabbit monoclonal [EPR14117(B)] to AACT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human AACT aa 1-100. The exact sequence is proprietary.
    Database link: P01011

  • Positive control

    • Human plasma and colon cancer lysates; Human liver and placenta tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab180492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP 1/70.

Target

  • Function

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

  • Tissue specificity

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

  • Involvement in disease

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].

  • Sequence similarities

    Belongs to the serpin family.

  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • alpha 1 Antichymotrypsin antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin family A member 3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA3 antibody
      see all

    Images

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Western blot analysis of  Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.

    • Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492)

    References (1)

    Publishing research using ab180492? Please let us know so that we can cite the reference in this datasheet.

    ab180492 has been referenced in 1 publication.

    • Li Y  et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). PubMed: 29399139

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