Recombinant Anti-AACT antibody [EPR14117(B)] (ab180492)
![Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-211947-anti-aact-antibody-epr14117b-western-blot.jpg "Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14117(B)] to AACT
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AACT antibody [EPR14117(B)]
See all AACT primary antibodies -
Description
Rabbit monoclonal [EPR14117(B)] to AACT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma and colon cancer lysates; Human liver and placenta tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14117(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
|
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| IP |
1/70.
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| Notes |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa). |
|
IP
1/70. |
Target
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Function
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
Tissue specificity
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
Involvement in disease
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 12 Human
- Omim: 107280 Human
- SwissProt: P01011 Human
- Unigene: 534293 Human
- Unigene: 710488 Human
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Alternative names
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
see all
Images
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Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg
Secondary
Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-211948-anti-aact-antibody-epr14117b-western-blot.jpg)
Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer lysate at 20 µg
Secondary
Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-211949-anti-aact-antibody-epr14117b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-211951-anti-aact-antibody-epr14117b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-211952-anti-aact-antibody-epr14117b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)Western blot analysis of Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.
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![Anti-AACT antibody [EPR14117(B)] (ab180492)](https://www.abcam.com/ps/products/180/ab180492/Images/ab180492-6-benefits-of-recombinant-antibodies.png)
Anti-AACT antibody [EPR14117(B)] (ab180492)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab180492 has been referenced in 1 publication.
- Li Y et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). PubMed: 29399139
