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  1. Link

    aact-antibody-epr14117b-ab180492.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Proteases
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RecombinantRabMAb

Recombinant Anti-AACT antibody [EPR14117(B)] (ab180492)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Anti-AACT antibody [EPR14117(B)] (ab180492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14117(B)] to AACT
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-AACT antibody [EPR14117(B)]
    See all AACT primary antibodies
  • Description

    Rabbit monoclonal [EPR14117(B)] to AACT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human plasma and colon cancer lysates; Human liver and placenta tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14117(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Associated products

  • Alternative Versions

    • Anti-AACT antibody [EPR14117(B)] - BSA and Azide free (ab250195)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human AACT protein (ab182695)
  • Related Products

    • Recombinant Human AACT protein (ab152025)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP
1/70.
Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP
1/70.

Target

  • Function

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificity

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in disease

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01011 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12 Human
    • Omim: 107280 Human
    • SwissProt: P01011 Human
    • Unigene: 534293 Human
    • Unigene: 710488 Human
    • Alternative names

      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • alpha 1 Antichymotrypsin antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin family A member 3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA3 antibody
      see all

    Images

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Western blot analysis of  Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.

    • Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab180492? Please let us know so that we can cite the reference in this datasheet.

    ab180492 has been referenced in 1 publication.

    • Li Y  et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). PubMed: 29399139

    Customer reviews and Q&As

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