Recombinant
RabMAb

Recombinant Anti-AACT antibody [EPR14117(B)] (ab180492)

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Overview

  • Product name

    Anti-AACT antibody [EPR14117(B)]
    See all AACT primary antibodies

  • Description

    Rabbit monoclonal [EPR14117(B)] to AACT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human AACT aa 1-100. The exact sequence is proprietary.
    Database link: P01011

  • Positive control

    • Human plasma and colon cancer lysates; Human liver and placenta tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP 1/70.

Target

  • Function

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

  • Tissue specificity

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

  • Involvement in disease

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].

  • Sequence similarities

    Belongs to the serpin family.

  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • alpha 1 Antichymotrypsin antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin family A member 3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA3 antibody
      see all

    Images

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Western blot analysis of  Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.

    References

    This product has been referenced in:

    • Li Y  et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). Read more (PubMed: 29399139) »
    See 1 Publication for this product

    Publishing research using ab180492? Please let us know so that we can cite the reference in this datasheet.

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