Overview

  • Product name

    Anti-AACT antibody [EPR5905]
    See all AACT primary antibodies
  • Description

    Rabbit monoclonal [EPR5905] to AACT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human AACT aa 250-450. The exact sequence is proprietary.

  • Positive control

    • WB: Human plasma and colon cancer lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 47 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
    • Tissue specificity

      Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
    • Involvement in disease

      Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
    • Sequence similarities

      Belongs to the serpin family.
    • Domain

      The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • alpha 1 Antichymotrypsin antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin family A member 3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA3 antibody
      see all

    Images

    • All lanes : Anti-AACT antibody [EPR5905] (ab129194) at 1/10000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    This product has been referenced in:

    • Valko PO  et al. In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study. J Sleep Res N/A:e12721 (2018). Read more (PubMed: 29961995) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    ELISA
    Sample
    Human Serum (secretome)
    Specification
    secretome
    Blocking step
    Blocking Buffer - ELISA Accessory Pack (ab210905) as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Type
    Sandwich (Capture)

    Dr. Marcia Ivonne Pena Paz

    Verified customer

    Submitted Feb 05 2018

    Question
    Answer


    We tested human plasma in IP with ab129194. Please see the attached file.

    Read More

    Question
    Answer

    We used New Zealand White Rabbits for production of this rabbit monoclonal antibody.

    Read More

    Question
    Answer

    Thank you for contacting Abcam.

    Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

    Read More

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