Overview

  • Product name

    AACT overexpression 293T lysate (whole cell)
  • Description

    AACT overexpression lysate
  • General notes

    ab94078 is a 293T cell transfected lysate in which Human AACT has been transiently over-expressed using a pCMV-AACT plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Research areas

  • Background

    Disease: Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. Domain: The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Similarity: Belongs to the serpin family. Tissue specificity: Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

Applications

Our Abpromise guarantee covers the use of ab94078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94078 at 15μg/lane on an SDS-PAGE gel.
  • All lanes : Anti-AACT antibody (ab54693) at 1/500 dilution

    Lane 1 : alpha 1 Antichymotrypsin 293T Transfected Lysate - Positive Control (ab94078)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94078 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab94078.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up