Anti-AADACL1 antibody (ab224127)
Key features and details
- Rabbit polyclonal to AADACL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AADACL1 antibody -
Description
Rabbit polyclonal to AADACL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human AADACL1 aa 200-350.
Database link: Q6PIU2 -
Positive control
- WB: Human cell line CAPAN-2; IHC-P: Human hippocampus tissue; ICC: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration. -
Tissue specificity
Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines. -
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. Microsome. - Information by UniProt
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Database links
- Entrez Gene: 534212 Cow
- Entrez Gene: 57552 Human
- Omim: 613234 Human
- SwissProt: Q1JQE6 Cow
- SwissProt: Q6PIU2 Human
- SwissProt: Q5R8Y5 Orangutan
- Unigene: 444099 Human
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Alternative names
- Arylacetamide deacetylase like 1 antibody
- Arylacetamide deacetylase-like 1 antibody
- NCEH antibody
see all
Images
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Anti-AADACL1 antibody (ab224127) at 0.4 µg/ml + Human cell line CAPAN-2
Predicted band size: 46 kDa -
Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224127 has not yet been referenced specifically in any publications.