Key features and details
- Rabbit polyclonal to AADACL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AADACL1 antibody
See all AADACL1 primary antibodies
DescriptionRabbit polyclonal to AADACL1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
- WB: AADACL1 over-expression - HEK-293T whole cell lysate; IHC-P: Human hippocampus tissue; ICC: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionHydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
Tissue specificityExpressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.
Sequence similaritiesBelongs to the 'GDXG' lipolytic enzyme family.
Cellular localizationMembrane. Microsome.
- Information by UniProt
- Arylacetamide deacetylase like 1 antibody
- Arylacetamide deacetylase-like 1 antibody
- NCEH antibody
All lanes : Anti-AADACL1 antibody (ab224127) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : AADACL1 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 46 kDa
Immunohistochemical analysis of human hippocampus tissue labeling AADACL1 in the cytoplasm of neuronal cells with ab224127 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling AADACL1 (green) in the cytoplasm and Golgi apparatus with ab224127 at 2 µg/ml.
ab224127 has not yet been referenced specifically in any publications.