Overview

  • Product name

  • Description

    Rabbit polyclonal to AAK1
  • Host species

    Rabbit
  • Specificity

    ab59740 recognizes 95kDa AAK1.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 850 of human AAK1.

  • Positive control

    • Mouse tissue lysate and rat kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab59740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAK 1 antibody
    • Aak1 antibody
    • AAK1_HUMAN antibody
    • Adaptor associated kinase 1 antibody
    • Adaptor-associated kinase 1 antibody
    • AP2 associated kinase 1 antibody
    • AP2 associated protein kinase 1 antibody
    • AP2-associated protein kinase 1 antibody
    • DKFZp686F03202 antibody
    • DKFZp686K16132 antibody
    • FLJ23712 antibody
    • FLJ25931 antibody
    • FLJ31060 antibody
    • FLJ42882 antibody
    • FLJ45252 antibody
    • KIAA1048 antibody
    • MGC138170 antibody
    • MGC164568 antibody
    • MGC164570 antibody
    see all

Images

References

This product has been referenced in:

  • Hu J  et al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009). Read more (PubMed: 19412185) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thanks for your phone call and for letting us know about the trouble with ab59740. As we discussed, I am sending a free of charge vial of ab77082 as a replacement, which should arrive late next week. If you have any Western blot images using ab59740, they will be very helpful in our investigation of the problem. Please let me know if you have any questions or if there is anything else that we can do for you.

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