Key features and details
- Rabbit polyclonal to AAK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-AAK1 antibody
See all AAK1 primary antibodies
DescriptionRabbit polyclonal to AAK1
Specificityab59740 recognizes 95kDa AAK1.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide surrounding amino acid 850 of human AAK1.
- Mouse tissue lysate and rat kidney lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab59740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).|
FunctionPhosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localizationMembrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
- Information by UniProt
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
All lanes : Anti-AAK1 antibody (ab59740) at 0.5 µg/ml
Lane 1 : Mouse tissue lysate
Lane 2 : Rat kidney tissue lysate
Predicted band size: 94 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
ab59740 has been referenced in 1 publication.
- Hu J et al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009). PubMed: 19412185