Anti-AAK1 antibody (ab77082)

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Overview

  • Product name

    Anti-AAK1 antibody
    See all AAK1 primary antibodies

  • Description

    Rabbit polyclonal to AAK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic 20 amino acid peptide from near the amino terminus of human AAK1 (NP_055726).

  • Positive control

    • Rat spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated.

  • Cellular localization

    Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AAK 1 antibody
    • Aak1 antibody
    • AAK1_HUMAN antibody
    • Adaptor associated kinase 1 antibody
    • Adaptor-associated kinase 1 antibody
    • AP2 associated kinase 1 antibody
    • AP2 associated protein kinase 1 antibody
    • AP2-associated protein kinase 1 antibody
    • DKFZp686F03202 antibody
    • DKFZp686K16132 antibody
    • FLJ23712 antibody
    • FLJ25931 antibody
    • FLJ31060 antibody
    • FLJ42882 antibody
    • FLJ45252 antibody
    • KIAA1048 antibody
    • MGC138170 antibody
    • MGC164568 antibody
    • MGC164570 antibody
    see all

Images

  • Western blot - Anti-AAK1 antibody (ab77082)
    Western blot - Anti-AAK1 antibody (ab77082)
    Lane 1 : Anti-AAK1 antibody (ab77082) at 1 µg/ml
    Lane 2 : Anti-AAK1 antibody (ab77082) at 2 µg/ml

    All lanes : A-20 lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 94 kDa
    Observed band size: 94 kDa

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab77082? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Could you tell me the immunogen sequence? If this is proprietary, could you tell me if itoverlaps withthe catalytic domain (amino acids 46-315)? I need to know this information for a co-IP that I would like to perform.

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Answer

Thank you for your call today and for your question about ab77082.

I have heard back from the lab, and though the exact immunogen sequence is considered proprietary, it does fall between amino acids 181 and 240. This region is within the catalytic domain, which is amino acids 46-315 according to UniProt.

http://www.uniprot.org/uniprot/Q2M2I8

I'm assuming that you would like to co-IP AAK1 bound to another protein at the catalytic domain? This antibody does not sound like it would work in such an assay, but there may a different AAK1 antibody that would work. Ab59740 is specific for a more C-terminal region, though it has not been tested in IP:

https://www.abcam.com/AAK1-antibody-ab59740.html

Please let me know if you have any questions or if there is anything else that I might be able to help you find.

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