Anti-AAK1 antibody (ab77082)

Submit a review Q&A (1)References (1)
Western blot - Anti-AAK1 antibody (ab77082)

    Key features and details

    • Rabbit polyclonal to AAK1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-AAK1 antibody
      See all AAK1 primary antibodies

    • Description

      Rabbit polyclonal to AAK1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Synthetic 20 amino acid peptide from near the amino terminus of human AAK1 (NP_055726).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab77082 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.
    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.

    Target

    • Function

      Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • AAK 1 antibody
        • Aak1 antibody
        • AAK1_HUMAN antibody
        • Adaptor associated kinase 1 antibody
        • Adaptor-associated kinase 1 antibody
        • AP2 associated kinase 1 antibody
        • AP2 associated protein kinase 1 antibody
        • AP2-associated protein kinase 1 antibody
        • DKFZp686F03202 antibody
        • DKFZp686K16132 antibody
        • FLJ23712 antibody
        • FLJ25931 antibody
        • FLJ31060 antibody
        • FLJ42882 antibody
        • FLJ45252 antibody
        • KIAA1048 antibody
        • MGC138170 antibody
        • MGC164568 antibody
        • MGC164570 antibody
        see all

      Images

      • Western blot - Anti-AAK1 antibody (ab77082)
        Western blot - Anti-AAK1 antibody (ab77082)
        Lane 1 : Anti-AAK1 antibody (ab77082) at 1 µg/ml
        Lane 2 : Anti-AAK1 antibody (ab77082) at 2 µg/ml

        All lanes : A-20 lysate

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 94 kDa
        Observed band size: 94 kDa

      References (1)

      Publishing research using ab77082? Please let us know so that we can cite the reference in this datasheet.

      ab77082 has been referenced in 1 publication.

      • Loi M  et al. MHC Class I Internalization via Autophagy Proteins. Methods Mol Biol 1880:455-477 (2019). PubMed: 30610715

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Could you tell me the immunogen sequence? If this is proprietary, could you tell me if itoverlaps withthe catalytic domain (amino acids 46-315)? I need to know this information for a co-IP that I would like to perform.

      Read More
      Answer

      Thank you for your call today and for your question about ab77082.

      I have heard back from the lab, and though the exact immunogen sequence is considered proprietary, it does fall between amino acids 181 and 240. This region is within the catalytic domain, which is amino acids 46-315 according to UniProt.

      http://www.uniprot.org/uniprot/Q2M2I8

      I'm assuming that you would like to co-IP AAK1 bound to another protein at the catalytic domain? This antibody does not sound like it would work in such an assay, but there may a different AAK1 antibody that would work. Ab59740 is specific for a more C-terminal region, though it has not been tested in IP:

      https://www.abcam.com/AAK1-antibody-ab59740.html

      Please let me know if you have any questions or if there is anything else that I might be able to help you find.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com