Overview

  • Product name

    Anti-AAMP antibody [EPR12369]
    See all AAMP primary antibodies
  • Description

    Rabbit monoclonal [EPR12369] to AAMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AAMP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13685

  • Positive control

    • WB: Fetal liver, HUVEC, 375, MCF7, Human brain, Mouse brain, Mouse heart, and Rat brain lysates; FC: MCF7 cells; ICC/IF: A375 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12369
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
ICC/IF 1/200.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a heparin-binding domain and mediates heparin-sensitive cell adhesion.
  • Cellular localization

    Cell membrane. Cytoplasm
  • Database links

  • Alternative names

    • AAMP antibody
    • Angio associated migratory cell protein antibody

Images

  • All lanes : Anti-AAMP antibody [EPR12369] (ab178691) at 1/1000 dilution (Purified)

    Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : Human brain lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Mouse heart lysates
    Lane 6 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling AAMP with Purifiedab178691 at 1/200 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling AAMP with purified ab178691 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • All lanes : Anti-AAMP antibody [EPR12369] (ab178691) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal liver lysate
    Lane 2 : HUVEC lysate
    Lane 3 : A375 lysate
    Lane 4 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 47 kDa

  • Flow cytometric analysis of permeabilized MCF7 cells labeling AAMP with unpurified ab178691 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

References

ab178691 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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