Anti-AASDHPPT antibody (ab235376)
Key features and details
- Rabbit polyclonal to AASDHPPT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AASDHPPT antibody -
Description
Rabbit polyclonal to AASDHPPT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human AASDHPPT aa 1 to the C-terminus.
Database link: Q9NRN7-1 -
Positive control
- WB: HepG2 whole cell lysate. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 36 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN. -
Tissue specificity
Detected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney. -
Sequence similarities
Belongs to the P-Pant transferase superfamily. AcpS family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 60496 Human
- Entrez Gene: 67618 Mouse
- Entrez Gene: 100174162 Orangutan
- Entrez Gene: 300328 Rat
- Omim: 607756 Human
- SwissProt: Q9NRN7 Human
- SwissProt: Q9CQF6 Mouse
- SwissProt: Q5NVE1 Orangutan
see all -
Alternative names
- 4' phosphopantetheinyl transferase antibody
- 4'-phosphopantetheinyl transferase antibody
- AASD PPT antibody
see all
Images
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Anti-AASDHPPT antibody (ab235376) at 1/200 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa -
Paraffin-embedded human testis tissue stained for AASDHPPT with ab235376 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235376 has not yet been referenced specifically in any publications.