Anti-AATF/DED antibody [3C7] (ab219073)
Key features and details
- Mouse monoclonal [3C7] to AATF/DED
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-AATF/DED antibody [3C7]
See all AATF/DED primary antibodies -
Description
Mouse monoclonal [3C7] to AATF/DED -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human AATF/DED aa 150-250. NCBI Accession No. AAH00591.
Database link: Q9NY61 -
Positive control
- Human kidney tissue; HeLa cells; HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3C7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. -
Tissue specificity
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus. -
Sequence similarities
Belongs to the AATF family. -
Post-translational
modificationsHyperphosphorylated during the G1/S phase transition. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 26574 Human
- Omim: 608463 Human
- SwissProt: Q9NY61 Human
- Unigene: 195740 Human
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Alternative names
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
see all
Images
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Immunofluorescent analysis of HeLa cells labeling AATF/DED using ab219073 at 10 µg/mL.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling AATF/DED using ab219073 at 10 µg/ml.
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Anti-AATF/DED antibody [3C7] (ab219073) + HeLa cell lysate
Predicted band size: 63 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab219073 has not yet been referenced specifically in any publications.