Key features and details
- Mouse monoclonal [3C7] to AATF/DED
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-AATF/DED antibody [3C7]
See all AATF/DED primary antibodies
DescriptionMouse monoclonal [3C7] to AATF/DED
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human kidney tissue; HeLa cells; HeLa cell lysate.
This product was previously labelled as AATF
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab219073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Sequence similaritiesBelongs to the AATF family.
modificationsHyperphosphorylated during the G1/S phase transition.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
Immunofluorescent analysis of HeLa cells labeling AATF/DED using ab219073 at 10 µg/mL.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling AATF/DED using ab219073 at 10 µg/ml.
Anti-AATF/DED antibody [3C7] (ab219073) + HeLa cell lysate
Predicted band size: 63 kDa
ab219073 has not yet been referenced specifically in any publications.