• Product name

  • Description

    Rabbit polyclonal to AATF/DED
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human AATF/DED (C terminal).

  • Positive control

    • Human small intestine cell lysate.
  • General notes

     This product was previously labelled as AATF




Our Abpromise guarantee covers the use of ab39631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.


  • Function

    May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • Tissue specificity

    Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • Sequence similarities

    Belongs to the AATF family.
  • Post-translational

    Hyperphosphorylated during the G1/S phase transition.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • BFR2 antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all


  • Immunofluorescence of AATF/DED in Human Small Intestine cells using ab39631 at 20 ug/ml.

  • Lane 1 : Anti-AATF/DED antibody (ab39631) at 0.5 µg/ml
    Lane 2 : Anti-AATF/DED antibody (ab39631) at 1 µg/ml
    Lane 3 : Anti-AATF/DED antibody (ab39631) at 2 µg/ml

    All lanes : Human small intestine cell lysate

    Lysates/proteins at 15 µg per lane.

    All lanes : anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size: 62 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

    (Lanes read from left to right, 1, 2, 3.)
  • ab39631 at 10µg/ml staining AATF/DED in small intestine by IHC


This product has been referenced in:

  • Li Z  et al. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4. Oncol Rep 38:2705-2716 (2017). Read more (PubMed: 29048629) »
  • Niida H  et al. Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo. EMBO J 29:3558-70 (2010). Read more (PubMed: 20834228) »
See all 2 Publications for this product

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