Product nameAnti-AATF/DED antibody
See all AATF/DED primary antibodies
DescriptionRabbit polyclonal to AATF/DED
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human AATF/DED (C terminal).
- Human small intestine cell lysate.
This product was previously labelled as AATF
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Sequence similaritiesBelongs to the AATF family.
modificationsHyperphosphorylated during the G1/S phase transition.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
Immunofluorescence of AATF/DED in Human Small Intestine cells using ab39631 at 20 ug/ml.
Lane 1 : Anti-AATF/DED antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF/DED antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF/DED antibody (ab39631) at 2 µg/ml
All lanes : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
(Lanes read from left to right, 1, 2, 3.)
ab39631 at 10µg/ml staining AATF/DED in small intestine by IHC
This product has been referenced in:
- Li Z et al. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4. Oncol Rep 38:2705-2716 (2017). Read more (PubMed: 29048629) »
- Niida H et al. Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo. EMBO J 29:3558-70 (2010). Read more (PubMed: 20834228) »