Key features and details
- Rabbit polyclonal to AATK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-AATK antibody
DescriptionRabbit polyclonal to AATK
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human AATK aa 1216-1374 (C terminal). (Expressed in E.coli).
LLSETFCEDLERKKKAVSFFDDVTVYLFDQESPTRELGEPFPGAKESPPT FLRGSPGSPSAPNRPQQADGSPNGSTAEEGGGFAWDDDFPLMTAKAAFAM ALDPAAPAPAAPTPTPAPFSRFTVSPAPTSRFSITHVSDSDAESKRGPEA GAGGESKEA
Database link: Q6ZMQ8
- WB: Recombinant human AATK protein; Mouse, rat and pig brain lysates. IHC-P: Human kidney, liver, stomach cancer, prostate gland and pancreatic cancer tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231529 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 145 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay be involved in neuronal differentiation.
Tissue specificityExpressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated by CDK5.
Cellular localizationMembrane. Cytoplasm. Cytoplasm > perinuclear region. Predominantly perinuclear.
- Information by UniProt
- AATK antibody
- AATYK antibody
- AATYK1 antibody
Paraffin-embedded human pancreatic cancer tissue stained for AATK using ab231529 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate gland tissue stained for AATK using ab231529 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach cancer tissue stained for AATK using ab231529 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for AATK using ab231529 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for AATK using ab231529 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-AATK antibody (ab231529) at 3 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Pig brain lysate
All lanes : HRP-linked guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 145 kDa
Anti-AATK antibody (ab231529) at 3 µg/ml + Recombinant human AATK protein.
HRP-linked guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 145 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231529 has not yet been referenced specifically in any publications.