Recombinant human MELK protein (ab102551)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
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Product name
Recombinant human MELK protein
See all MELK proteins and peptides -
Biological activity
The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
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Purity
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
61 kDa including tags -
Amino acids
1 to 340
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Specifications
Our Abpromise guarantee covers the use of ab102551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AI327312
- hMELK
- HPK 38
see all -
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab102551 has not yet been referenced specifically in any publications.