Recombinant human Casein Kinase 1 delta/CSNK1D protein (ab103955)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
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Product name
Recombinant human Casein Kinase 1 delta/CSNK1D protein
See all Casein Kinase 1 delta/CSNK1D proteins and peptides -
Biological activity
The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
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Purity
> 80 % Densitometry.
Purity was determined to be >80% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
72 kDa including tags -
Amino acids
1 to 415
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab103955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as Casein Kinase 1 delta.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Casein Kinase 1 delta
- Casein kinase I delta isoform
- Casein kinase I isoform delta
see all -
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
Tissue specificity
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
Involvement in disease
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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The specific activity of Casein Kinase 1 delta/CSNK1D (ab103955) was determined to be 77 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab103955
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SDS PAGE analysis of ab103955
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The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
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SDS-PAGE showing ab103955 at approximately 72kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab103955 has been referenced in 2 publications.
- Ibrahim H et al. Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation. Int J Mol Sci 22:N/A (2021). PubMed: 33917494
- Hanna-Addams S et al. CK1a, CK1d, and CK1e are necrosome components which phosphorylate serine 227 of human RIPK3 to activate necroptosis. Proc Natl Acad Sci U S A 117:1962-1970 (2020). PubMed: 31932442