Anti-Nkx2.5 antibody (ab106923)
Key features and details
- Goat polyclonal to Nkx2.5
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nkx2.5 antibody
See all Nkx2.5 primary antibodies -
Description
Goat polyclonal to Nkx2.5 -
Host species
Goat -
Specificity
ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
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Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Nkx2.5 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Mouse heart lysate; ICC: U-2 OS cells. IHC: Human heart.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
ICC |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Only suitable for human, not mouse samples. |
Notes |
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WB
Use a concentration of 1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Only suitable for human, not mouse samples. |
Target
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Function
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
Tissue specificity
Expressed only in the heart. -
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1482 Human
- Entrez Gene: 18091 Mouse
- Omim: 600584 Human
- SwissProt: P52952 Human
- SwissProt: P42582 Mouse
- Unigene: 54473 Human
- Unigene: 41974 Mouse
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Alternative names
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
Images
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All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Mouse heart lysate in RIPA buffer
Lane 3 : Rat heart lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunohistochemical analysis of human heart tissue stained for Nkx2.5 using ab106923 at 4µg/ml. Positive cytoplasmic staining shown.
Datasheets and documents
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Datasheet download
References (6)
ab106923 has been referenced in 6 publications.
- Zhu D et al. Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair. Nat Commun 12:1412 (2021). PubMed: 33658506
- Yu B et al. Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24:N/A (2021). PubMed: 34664684
- Chatterjee S & Chakravarti A A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease. Hum Mol Genet 28:3137-3147 (2019). PubMed: 31313802
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Liu H et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int 2016:2524092 (2016). PubMed: 26798354
- Mohammadi A et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J 17:288-95 (2015). Rat . PubMed: 26199907