Recombinant Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3282] to EN1 / Engrailed 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-EN1 / Engrailed 1 antibody [EPR3282]
See all EN1 / Engrailed 1 primary antibodies -
Description
Rabbit monoclonal [EPR3282] to EN1 / Engrailed 1 -
Host species
Rabbit -
Specificity
We have data to suggest no cross-reactivity with EN2/Engrailed 2, please contact us if you require further details/experimental data
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, SH-SY5Y, 293T and Human fetal brain lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3282 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). |
Target
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Sequence similarities
Belongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2019 Human
- Entrez Gene: 13798 Mouse
- Entrez Gene: 685360 Rat
- Omim: 131290 Human
- SwissProt: Q05925 Human
- SwissProt: P09065 Mouse
- Unigene: 271977 Human
- Unigene: 2657 Mouse
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Alternative names
- En 1 antibody
- EN1 antibody
- Engrailed 1 antibody
see all
Images
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All lanes : Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598) at 1/1000 dilution
Lane 1 : U87-MG lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
All lanes : Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108598 has been referenced in 2 publications.
- Shen J et al. MicroRNA-34c promotes neuronal recovery in rats with spinal cord injury through the C-X-C motif ligand 14/Janus kinase 2/signal transducer and activator of transcription-3 axis. Chin Med J (Engl) 133:2177-2185 (2020). PubMed: 32826607
- Wu SY et al. Opposing Functions of BRD4 Isoforms in Breast Cancer. Mol Cell 78:1114-1132.e10 (2020). PubMed: 32446320