Recombinant Anti-SENP1 antibody [EPR3844] (ab108981)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3844] to SENP1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SENP1 antibody [EPR3844]
See all SENP1 primary antibodies -
Description
Rabbit monoclonal [EPR3844] to SENP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HUVEC, Jurkat, Daudi and U87-MG cell lysates. IHC-P: Human testis tissue. ICC/IF: Jurkat cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3844 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
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ICC/IF | (3) |
1/100 - 1/500.
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WB | (4) |
1/1000 - 1/20000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/100 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/20. |
ICC/IF
1/100 - 1/500. |
WB
1/1000 - 1/20000. Predicted molecular weight: 73 kDa. |
IHC-P
1/100 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity. -
Tissue specificity
Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine. -
Sequence similarities
Belongs to the peptidase C48 family. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29843 Human
- Omim: 612157 Human
- SwissProt: Q9P0U3 Human
- Unigene: 603289 Human
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Alternative names
- SENP 1 antibody
- SENP1 antibody
- SENP1_HUMAN antibody
see all
Images
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Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution (purified) + Daudi cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunofluorescence staining of Jurkat cells with purified ab108981 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108981 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Immunohistochemical staining of paraffin embedded human testis with purified ab108981 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Intracellular Flow Cytometry analysis of HeLa cells labelling SENP1 with purified ab108981 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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Unpurified ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
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Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution (purified) + U87-MG cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysates
Lane 2 : HUVEC cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : Daudi cell lysates
Lane 5 : U87-MG cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa -
All lanes : Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution (unpurified)
Lane 1 : COS-1 cell lysate
Lane 2 : COS-1 cell lysate transfected with SENP1
Lane 3 : COS-1 cell lysate transfected with SENP1 mutant
Lysates/proteins at 20000 cells per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocked with 3% milk for 1 hour at 25°C.
Incubated with the primary antibody for 18 hours at 4°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab108981 has been referenced in 39 publications.
- Yu L et al. Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylation. Oxid Med Cell Longev 2022:8002566 (2022). PubMed: 35707278
- Yan L et al. SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation. Nat Commun 13:7153 (2022). PubMed: 36414671
- Shangguan X et al. SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis. Nat Commun 12:1812 (2021). PubMed: 33753739
- Li J et al. SENP1-mediated deSUMOylation of JAK2 regulates its kinase activity and platinum drug resistance. Cell Death Dis 12:341 (2021). PubMed: 33795649
- Hou G et al. SUMOylation of YTHDF2 promotes mRNA degradation and cancer progression by increasing its binding affinity with m6A-modified mRNAs. Nucleic Acids Res 49:2859-2877 (2021). PubMed: 33577677