Recombinant Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3101(2)] to Wnt2/IRP
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt2/IRP antibody [EPR3101(2)]
See all Wnt2/IRP primary antibodies -
Description
Rabbit monoclonal [EPR3101(2)] to Wnt2/IRP -
Host species
Rabbit -
Tested applications
Suitable for: WB, Indirect ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197186) -
Positive control
- WB: Jurkat, MCF-7,T47-D, and SKBR-3 cell lysates. Rat and mouse brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3101(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa).
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Indirect ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa). |
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Tissue specificity
Expressed in brain in the thalamus, in fetal and adult lung and in placenta. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7472 Human
- Entrez Gene: 22413 Mouse
- Entrez Gene: 114487 Rat
- Omim: 147870 Human
- SwissProt: P09544 Human
- SwissProt: P21552 Mouse
- Unigene: 567356 Human
- Unigene: 33653 Mouse
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Form
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. -
Alternative names
- Int 1 related protein antibody
- Int-1-like protein 1 antibody
- Int-1-related protein antibody
see all
Images
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All lanes : Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : SKBR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Representative standard curve using ab109222
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Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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All lanes : Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysates
Lane 2 : MCF-7 cell lysates
Lane 3 : T47-D cell lysates
Lane 4 : SKBR-3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab109222 has been referenced in 14 publications.
- Yu H et al. SNHG12 promotes carcinogenesis of human renal cell cancer via functioning as a competing endogenous RNA and sponging miR-30a-3p. J Cell Mol Med 25:4696-4708 (2021). PubMed: 33787057
- Xie B et al. MicroRNA-1246 by Targeting AXIN2 and GSK-3ß Overcomes Drug Resistance and Induces Apoptosis in Chemo-resistant Leukemia Cells. J Cancer 12:4196-4208 (2021). PubMed: 34093820
- Zou S et al. ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition. J Biochem Mol Toxicol 34:e22454 (2020). PubMed: 31981282
- Guan G et al. Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin-induced in vitro ototoxicity in HEI-OC1 auditory cells using gene expression profiling. Mol Med Rep 22:5053-5068 (2020). PubMed: 33174043
- Chen Q et al. Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression. BMC Mol Cell Biol 21:80 (2020). PubMed: 33167868