Recombinant Anti-NRG3 antibody [EPR4148] (ab109256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4148] to NRG3
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NRG3 antibody [EPR4148]
See all NRG3 primary antibodies -
Description
Rabbit monoclonal [EPR4148] to NRG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fetal brain, SH-SY5Y, C6, mouse brain and rat brain lysates. Flow Cyt (intra): Permeabilized C6 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4148 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. -
Tissue specificity
Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Belongs to the neuregulin family.
Contains 1 EGF-like domain. -
Developmental stage
Isoform 3 is expressed in fetal brain but not in other fetal tissues. -
Domain
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
Post-translational
modificationsProteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated. -
Cellular localization
Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form. - Information by UniProt
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Database links
- Entrez Gene: 10718 Human
- Entrez Gene: 18183 Mouse
- Entrez Gene: 498596 Rat
- Omim: 605533 Human
- SwissProt: P56975 Human
- SwissProt: O35181 Mouse
- Unigene: 125119 Human
- Unigene: 258705 Mouse
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Alternative names
- HRG3 antibody
- Neuregulin 3 antibody
- Neuregulin 3 like polypeptide antibody
see all
Images
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All lanes : Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : C6 lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeabilized C6 (Rat glial tumor cell line) cells using ab109256 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109256 has been referenced in 2 publications.
- Wang F et al. Resveratrol ameliorates depressive disorder through the NETRIN1-mediated extracellular signal-regulated kinase/cAMP signal transduction pathway. Mol Med Rep 17:4611-4618 (2018). PubMed: 29328454
- Liu Y et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). PubMed: 27449290